TITLE:
Associations between Dietary Intake of Fruits and Vegetables in Relation to Urinary Estrogen DNA Adduct Ratio
AUTHORS:
Kerryn W. Reding, Muhammad Zahid, Ercole Cavalieri, Eleanor G. Rogan, Brianne S. Raccor, Charlotte Atkinson, Mellissa Yong, Katherine M. Newton, Johanna W. Lampe
KEYWORDS:
Breast Cancer, Nutritional Assessment, Biomarker, Estrogens
JOURNAL NAME:
Open Journal of Preventive Medicine,
Vol.4 No.6,
June
11,
2014
ABSTRACT: Background:
Estrogen exposure plays a role in breast cancer (BC) development. A novel
estrogen biomarker, the estrogen DNA adduct (EDA) ratio, was shown to be
elevated in women at high-risk of BC and among BC cases. Modifiable factors may
impact the EDA ratio, with studies demonstrating that resveratrol reduces EDA
ratio in vitro. We sought to examine
the hypothesis that dietary intake of fruits and vegetables is inversely
associated with EDA ratio. Methods: This analysis was conducted in 53
pre-menopausal, healthy women aged 40 - 45 years from a cross-sectional study
in which participants provided first-void urine samples and 3-day food records.
Urine samples were analyzed using ultra performance liquid chromatography/tandem
mass spectrometry. The EDA ratio was calculated as the estrogen-DNA adducts divided
by estrogen metabolites and conjugates. A trend test was used to assess associations
between tertiles of dietary intake using linear regression. Results: After
adjustment for age, total energy, percent adiposity, serum estradiol and
estrone-sulfate, we observed inverse associations of EDA ratio with
carbohydrate consumption (P = 0.01)
and vegetable intake (P = 0.01). EDA
ratio was inversely associated with 5 botanical groups (Chenopodiaceae: P = 0.02; Umbelliferae: P = 0.03; Compositae: P = 0.01; Ericaceae: P = 0.01; Musaceae: P = 0.03) but not fruit intake overall. Conclusion: Although these
data require replication before conclusions are drawn, this report suggests an
inverse association between vegetable and carbohydrate consumption and EDA
ratio. Impact: While more information is still needed, these findings suggest a
link between dietary intake and a biomarker that is both associated with high-risk
BC status and associated with modifiable factors.