TITLE:
Meizothrombin Preparation and Its Role in Fibrin Formation and Platelet Aggregation
AUTHORS:
Dar’ya S. Korolova, Tamara M. Chernyshenko, Olga V. Gornytska, Volodymyr O. Chernyshenko, Tatiana N. Platonova
KEYWORDS:
Prothrombin, Meizothrombin, Platelets, Fibrin Polymerization
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.7,
June
5,
2014
ABSTRACT: Meizothrombin (MT) is one of prothrombin derivatives which appears in haemostasis activation area. However, its role
in haemostasis regulation isn’t clear. We studied the role of MT in fibrin
formation, platelet activation and aggregation. A new effective method of
obtaining MT from native human prothrombin was developed using immobilised
prothrombin activator from Echis
multisquamatis venom. The protein was stable and electrophoretically pure.
Platelet-rich plasma for aggregation study and gel-sieved platelets for
flow-cytometry were separated from blood of healthy donors. It was shown that
MT transformed fibrinogen to fibrin and activated clotting factor XIII. MT
didn’t activate gel-sieved intact platelets, but in platelet-rich plasma,
increased platelet aggregation induced by ADP, collagen and adrenalin.