TITLE:
Anticancer Effect of Ginseng Leaves Crude Polysaccharides on Human Hepatoma Cell SMMC-7721
AUTHORS:
Xiaozhen Fu, Dan Shi, Chao Qu, Guangying Zhong, Wei Zou, Jing Liu
KEYWORDS:
Anticancer, Ginseng Leaves Crude Polysaccharides (GLCP), Caveolin-1, Autophagy, Apoptosis
JOURNAL NAME:
Chinese Medicine,
Vol.5 No.2,
June
5,
2014
ABSTRACT: Background: Polysaccharides extracted from the medicinal
herbs are known to possess antitumor effects. Although there is a significant
number of evidences implicated on the beneficial effects of the ginseng leaves
ginsenoside with diverse associated mechanisms, reports on the anticancer by
the ginseng leaves crude polysaccharides (GLCP) are not
sufficient. Experiments were carried out to unravel the anticancer effects of
GLCP. Methods: Cells were treated with GLCP (0.5 - 2 mg/ml) for
48h. MTT method was used to detect the cell viability. Western blot and flow
cytometry were used to detect apoptotic rate. Western blot and acridine orange
staining were used to detect the cell autophagy. Results: Compared with the
normal human liver cell (Chang liver), GLCP (1.5 - 2 mg/ml) significantly
reduction cell viability, promote apoptosis-related
proteins expression, promote cell apoptosis and autophagy in
SMMC-7721 cells. But caveolin-1 gene silencing
could inhibit the anticancer effect of GLCP. Conclusions: These data suggest
that GLCP promote autophagy and apoptosis in human hepatoma cell SMMC-7721.
We speculate that its mechanism may be associated with the caveolin-1 which is an essential structural molecule of caveolae. Although the effect of GLCP
inhibited of liver cancer is not very strong, we are
more interested in the GLCP which plays a tumor suppressor role in health care.