TITLE:
Cell Proliferation Ability of Mouse Fibroblast-Like Cells and Osteoblast-Like Cells on a Ti-6Al-4V Alloy Film Produced by Selective Laser Melting
AUTHORS:
Mayu Kawase, Tatsuhide Hayashi, Masaki Asakura, Akimichi Mieki, Hironari Fuyamada, Masahiro Sassa, Shizuka Nakano, Masashi Hagiwara, Toru Shimizu, Tatsushi Kawai
KEYWORDS:
Selective Laser Melting (SLM), Ti-6Al-4V Film, Mouse Fibroblast-Like Cell, Mouse Osteoblast-Like Cell, Cell Compatibility
JOURNAL NAME:
Materials Sciences and Applications,
Vol.5 No.7,
May
29,
2014
ABSTRACT: Successful regeneration of tissues and organs relies on the application
of suitable substrates or scaffolds in scaffold-based regenerative medicine. In
this study, Ti-6Al-4V alloy films (Ti alloy film) were produced using a three-dimensional
printing technique called Selective Laser Melting (SLM), which is one of the
metal additive manufacturing techniques. The thickness of produced Ti alloy
film was approximately 250 μm. The laser-irradiated surface of Ti alloy film had
a relatively smooth yet porous surface. The non-irradiated surface was also
porous but also retained a lot of partially melted Ti-6Al-4V powder. Cell proliferation
ability of mouse fibroblast-like cells (L929 cells) and mouse osteoblast-like
cells (MC3T3-E1 cells) on both the surfaces of Ti alloy film was examined using
WST assay. Both L929 and MC3T3-E1 cells underwent cell proliferation during the
culture period. These results indicate that selective laser melting is suitable
for producing a cell-compatible Ti-6Al-4V alloy film for biomaterials
applications.