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Salvioli, S., Monti, D., Lanzarini, C., Conte, M., Pirazzini, C., et al. (2013) Immune System, Cell Senescence, Aging and Longevity-Inflamm-Aging Reappraised. Current Pharmaceutical Design, 19, 1675-1679.

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• TITLE: The Rate of Aging of the Dunaliella viridis Teodor. Culture Depends on the Algae Pre-Adaptation to Copper Sulfate Toxic Effect

  AUTHORS: A. I. Bozhkov, M. K. Kovaleva, N. G. Menzyanova, Y. A. Kuznetsova

  KEYWORDS: Microalgae Dunaliella viridis, Adaptation to Copper Ions, Chronological and Replicative Aging, Epigenotype

  JOURNAL NAME: Advances in Aging Research, Vol.3 No.2, May 29, 2014

  ABSTRACT: Two sublines of microalgae Dunaliella viridis Teodor. were obtained: CuS—a subline sensitive to toxic concentration of copper ions and CuR—a subline resistant to toxic concentration of copper ions. The chronological aging of cultures was revealed in increase of DNA (polyploidization) and triacylglycerols (TAG) content in microalgae cells. The adaptation of D. viridis to toxic concentrations of copper ions resulted in formation of an adaptive epygenotype characterized by increased content of carbonylated proteins and decreased content of proline compared to the CuS-subline. The adaptation of D. viridis to toxic concentrations of copper ions resulted in the increase of the rate of chronological aging compared to the CuS-subline. Four subcultures with different rates of transplanting were obtained from each subline. The rate of replicative aging was shown to be dependent on the rate of chronological aging of subcultures (passage rate). The pre-adaptation of D. viridis to toxic concentrations of copper ions was accompanied by increase of accumulation rate of TAG and DNA in cells, which was interpreted as a sign of cellular aging during the progressive passages of the algae culture.