TITLE:
Molecular Identification of Shiga-Toxin Producing and Enteropathogenic Escherichia coli (STEC and EPEC) in Diarrheic and Healthy Young Alpacas
AUTHORS:
Lorena Mori, Rosa Perales, Jorge Rodríguez, Carlos Shiva, Ysabel Koga, Geraldine Choquehuanca, Cesar Palacios
KEYWORDS:
Shiga Toxin Producing Escherichia coli, Enteropathogenic Escherichia coli, Diarrhea, Young Alpacas
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.7,
May
26,
2014
ABSTRACT: Isolation and biochemical and molecular
identification of 303 strains of Escherichia
coli obtained from diarrheic and healthy young alpacas of Puno-Peru, were
realized. PCR amplification for 7 virulence factor genes associated with STEC,
STEC O157:H7, EPEC: sxt1, sxt2, rfbO157, fliCH7, hlyA, eae y bfp were determined. A total of 39 strains (12.88%) showed amplification for one or more of these
genes. Twenty three strains (59%) were classified as STEC and 16 strains (41%)
as EPEC. An 88.18% (34/39) of STEC and EPEC strains were obtained from healthy
alpacas and only 11.82% (5/39) from diarrheic alpacas considering this specie
as potential zoonotic reservoir of STEC and EPEC.