TITLE:
Preliminary Isolation and Antibacterial Activity of the Ethyl Acetate Extract of Prinsepia utilis Royle in Vitro
AUTHORS:
Zhonghui Pu, Zhongqiong Yin, Renyong Jia, Xu Song, Jiao Xu, Xiongqing Wang, Xiwen Chen, Minghua Luo
KEYWORDS:
Antibacterial Activity, Prinsepia utilis Royle, MIC, MBC
JOURNAL NAME:
Agricultural Sciences,
Vol.5 No.6,
May
23,
2014
ABSTRACT: Seven fractions were isolated from the ethyl acetate extract of Prinsepia utilis Royle by silica gel column chromatography. The antibacterial
activities determined by disc diffusion method in vitro, indicated that the
first and fourth fractions showed better antibacterial activity than the other fractions,
while the sixth and seventh fractions did not showed any antibacterial activity.
The diameters of the inhibition zone of first and fourth fractions were greater
than 10 mm against three standard strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC44102 and Salmonella sp. CMCC(B)50041) at
the concentration of 20 mg/ml. The first fraction was then repeatedly recrystallized
in acetone to yield a white snowflake-like compound A, the inhibition zones of which
were 14.03 mm, 11.54 mm and 12 mm, respectively. The Minimum Inhibitory Concentration
(MIC) and Minimum Bactericidal Concentration (MBC) were measured by broth dilution
method at the concentrations ranging from 20 to 0.313 mg/mL. The MIC and MBC values
of the first, fourth fractions and compound A were lower than that of oregano oil
(positive control) against S. aureus ATCC25923.