TITLE:
Application of Green Fluorescent Protein in Immunoassays
AUTHORS:
Seiichi Sakamoto, Yukihiro Shoyama, Hiroyuki Tanaka, Satoshi Morimoto
KEYWORDS:
GFP, Immunoassay, Fluobody
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.6,
May
23,
2014
ABSTRACT: Green fluorescent protein (GFP) is a protein
that emits green fluorescence when exposed to a radiation of ultraviolet
wavelength range, even without the addition of substrate and cofactor. Because
of such characteristics, the usage of GFP is widespread in both in vivo and in vitro applications. In addition, recent advances in
biotechnology have enabled GFP to be expressed in various hosts, including
bacteria, yeast, plants, animals, and even living-cells, for multiple purposes.
Currently, GFP is a subject of great interest in the analytical sciences,
especially in immunoassays for qualitative and quantitative analyses, when it
is fused with an antibody because of the high sensitivity of GFP and
antigen-binding specificity of antibodies. Recently the fluobody, which is a
fusion protein of GFP with single-chain variable fragment antibody (scFv), has
become a useful tool in various fields. We review here the applications of GFP
as fluobodies in immunoassays.