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Yahata, M., Kunitake, H., Yabuya, T., Yamashita, K., Kashihara, Y. and Komatsu, H. (2005) Production of a Doubled Haploid from a Haploid Pummelo Using Colchicine Treatment of Axillary Shoot Buds. Journal of the American Society for Horticultural Science, 130, 899-903.

has been cited by the following article:

• TITLE: In Vitro Induction of Polyploidy in Citrus reticulata Blanco

  AUTHORS: Doaa M. Abou Elyazid, Ali R. El-Shereif

  KEYWORDS: Colchicine, DNA, Mandarin, Polyploidy, Tetraploid

  JOURNAL NAME: American Journal of Plant Sciences, Vol.5 No.11, May 21, 2014

  ABSTRACT: Possibility of polyploidy induction by colchicine in “Balady” mandarins was investigated in vitro. Seeds were immersed in different concentrations of colchicine solutions (0.01%, 0.05%, 0.1% and 0.2%) for different durations (12, 24 and 48 hr), then cultured in vitro on MS medium at half strength. Seed survival percentage decreased by increasing colchicine concentration and duration of treatment showing the lowest percentage at 0.2% for 48 hr. The highest DNA content was recorded at 0.2% for 24 hr. Stomata No. per unit area was decreased by colchicine treatments; moreover stomata length and width were studied. The results indicated that colchicine treatment at 0.1% for 48 hr had the highest tetraploid induction efficiency percentage.