TITLE:
Comparative Analysis of Genetic Diversity among Cultivated Pigeonpea (Cajanus cajan (L) Millsp.) and Its Wild Relatives (C. albicans and C. lineatus) Using Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) Fingerprinting
AUTHORS:
Kusum Yadav, Sanjay Kumar Yadav, Anurag Yadav, Veda Prakash Pandey, Upendra Nath Dwivedi
KEYWORDS:
Genetic Diversity, RAPD, ISSR, Pigeonpea
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.11,
May
21,
2014
ABSTRACT: Genetic
relationships of 16 cultivars of pigeonpea (Cajanus
cajan (L) Millsp.) and its two wild relatives (C. albicans and C. lineatus)
from different parts of the India were analysed using 22 random amplified
polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs)
primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with
an average of 6.8 polymorphic band/primer. Cluster analysis based on these 151
RAPD markers revealed relatively low level (0.434 - 0.714) of genetic diversity
among cultivars and high level of diversity between cultivars and wild
relatives. Ten ISSR primers produced 100 bands across 16 cultivars and its wild relatives out of which 93 (93%) were
polymorphic with an average of 9.3 polymorphic band/primer. Cluster
analysis based on these 93 ISSR markers also revealed relatively higher level (0.328
- 0.827) of genetic diversity among cultivars as compared to RAPD markers. The
polymorphic markers obtained by both RAPD and ISSR primers were pooled and the
genetic diversity analysis based on these 244 markers was analysed. Jaccard’s
similarity coefficient obtained by pooled data revealed very narrow range (0.477
- 0.720) among cultivated and high range between cultivated and wild species C. albicans (0.240 - 0.331) and C. lineatus (0.163 - 0.193). In the
UPGMA based dendrogram the 16 cultivars were grouped into three distinct
clusters. Cluster I contained two genotypes, cluster II had six and cluster III
had eight genotypes. Principal components analysis (PCA) also resulted in
similar pattern as that of UPGMA based analysis. The first three PCs contributed
56.26%, 5.71% and 4.97% of variation, respectively, with cumulative variation
of the first three PCs was 66.96%. Both the markers and the combined data
revealed similar pattern with narrow diversity among cultivars and higher
diversity between cultivars and wild one, but the genetic diversity range
obtained by ISSR markers was relatively higher as compared to RAPD and pooled
data. Furthermore, both the markers also correlated the clustering of stress
resistant genotypes together. Cultivar Pusa-2002 possessed more diversity with
other genotypes in ISSR dendrogram.