TITLE:
Antioxidant Activity and Lipoxygenase Inhibitory Effect of Caralluma arabica and Related Polyphenolic Constituents
AUTHORS:
Mohammad Khasawneh, Hanan Mohamed Elwy, Nael Mohamed Fawzi, Alaaeldin Ahmed Hamza, Abdul Raheem Chevidenkandy, Ahmed H. Hassan
KEYWORDS:
Caralluma arabica, Antioxidants, Anticancer, MTT Assay
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.11,
May
19,
2014
ABSTRACT: Phytochemicals have
been of great interest as a source of natural antioxidants used for health
promotion, food preservation, food flavoring and cosmetics. In this research,
alcoholic extract from Caralluma arabica and different solvent fractions
were evaluated for their antioxidant, anti-inflammatory and anti-cancer
properties as well as their polyphenolic compositions. The total antioxidant
property was estimated by the ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
(ABTS+) and β-carotene bleaching tests. Among the ethanol extract and three
fractions, the ethyl acetate fraction showed the highest phenolic content (33.1
mg gallic acid/g) and the best antioxidant activity. The ascorbic acid
equivalent antioxidant capacities of the ethyl acetate fraction were 143.64, 381.3,
112.6 μmol/g in FRAP, ABTS and DPPH assays, respectively. This study verified
that the ethanol extract and ethyl acetate fraction from Caralluma arabica have strong antioxidant activity that was
correlated with their high level of phenolic content. Furthermore, the same
extract showed appreciable anti-inflammatory via Lipoxygenase inhibitory
activity (LOX), the IC50 values ranged from 11.2 - 30.77 μg/mL.
Moreover, Ethyl acetate fraction showed the strongest cytotoxic effect (IC50 = 87.55 μg/mL) against MCF-7 breast cancer cell line. These findings suggest
that Caralluma arabica may be considered as an interesting source of
antioxidants for nutraceutical industries.