TITLE:
Effects of Sodium Diclofenac on the Distribution of Fos Protein in Central Amygdala and Lateral Hypothalamus during Experimental Tooth Movement in Rats
AUTHORS:
Ana Paula R. Novaes, Amanda C. Desiderá, Glauce C. Nascimento, Christie R. A. Leite-Panissi
KEYWORDS:
Amygdala, Lateral Hypothalamus, Fos Protein, Orthodontic Movement, Sodium Diclofenac
JOURNAL NAME:
World Journal of Neuroscience,
Vol.4 No.2,
May
15,
2014
ABSTRACT: This study evaluated whether the administration of a NSAID, sodium diclofenac, can promote alterations in the expression of Fos protein in central amygdala (CEA) and the lateral hypothalamus (LH) after 6 h of experimental tooth movement with a controlled force of 70 g, applied to the superior central incisors of rats. Adult male rats were anesthetized and divided into four groups: Control, no orthodontic appliance (OA); OA activated with 70 g; OA activated with 70 g and pretreated with diclofenac sodium (5 mg/kg, intramuscular); and diclofenac sodium alone. Six hours after the onset of the experiment the rats were reanesthetized and perfused with 4% paraformaldehyde. The brains were removed and fixed, and sections containing the CEA and LH were processed for Fos protein immunohistochemistry. The results show that in the control group, intramuscular injection of a ketamine/xylazine mixture did not induce IR-Fos cells in the CEA or LH. However, in the 70 g group, IR-Fos was the strongest observed (P