TITLE:
Immobilization of Commercial Cellulase and Xylanase by Different Methods Using Two Polymeric Supports
AUTHORS:
Sheila Romo-Sánchez, Conrado Camacho, Héctor L. Ramirez, María Arévalo-Villena
KEYWORDS:
Cellulase, Crosslinking-Adsorption, Immobilization, Reticulation, Xylanase
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.6,
May
14,
2014
ABSTRACT: Industrial applications require enzymes highly
stable and economically viable in terms of reusability. Enzyme immobilization
is an exciting alternative to improve the stability of enzymatic processes. The immobilization of two commercial
enzymes is reported here (cellulase and xylanase) using three chemical
methods (adsorption, reticulation, and crosslinking-adsorption) and two
polymeric supports (alginate-chitin and chitosan-chitin). The optimal pH for
binding was 4.5 for cellulase and 5.0 for xylanase, and the optimal enzyme
concentrations were 170 μg/mL and 127.5 μg/mL respectively, being the chitosan and
the ideal support. In some cases, a low concentration of crosslinking agent
(glutaraldehyde) improved stability of the immobilization process. Biotechnological
characterization showed that the reusability of enzymes was the most striking
finding, particularly of immobilized cellulase using glutaraldehyde, which
after 19 cycles retained 64% activity. These results confirm the economic and
biotechnical advantages of enzyme immobilization for a range of industrial
applications.