TITLE:
In Vivo Preliminary Study on Bone Neoformation Behavior of Three Types of Calcium Phosphate Bioceramics
AUTHORS:
Nelson H. A. Camargo, Enori Gemelli, Aury N. de Moraes, Bruna D. da Costa, Nilson Oleskovicz, Ademar L. Dallabrida, Doughlas Regalin, Marcos Paulo Antunes de Lima
KEYWORDS:
Bioceramics; Microstructure; Bone Neoformation; Characterization
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.2 No.2,
April
29,
2014
ABSTRACT: Calcium phosphate microporous bioceramics
and biphasic compositions of hydroxyapatite and β-calcium phosphate, in the
form of microporous granular biomaterials, are research topics and present
themselves as potential orthopedic and biomedical applications in rebuilding
and repairing maxillofacial bones and tooth structure. This is associated with
the characteristics of microstructure, biocompatibility, bioactivity and bone
conductivity properties which these materials offer when applied in vivo or in
a simulation environment. This study aimed to assess the behavior of bone
neoformation of three types of calcium phosphate biomaterials in in vivo tests
with sheep within 60 and 90 days, with the help of a scanning electron
microscope. The biomaterials used were provided by the Group of Biomaterials at
the Santa Catarina State University. The in vivo tests were carried out by
generating, on sheep, tibial bone defects, three of which were filled with
biomaterial (one different biomaterial for each bone defect generated), whilst
the fourth received a bone fragment obtained during the generation of the
defect in question, to serve as a control group. The scanning electron
microscopy (SEM) technique was used for carrying out the preliminary
characterization studies so as to observe new bone formation and
osseointegration. The X-ray diffractometry (XRD) served as a support for the
characterization of crystalline phases. The results obtained are encouraging
and show that the biomaterials presented good performance in the process of
bone formation, biomaterial osseointegration by a new tissue and bone mineralization.