TITLE:
Bi-Enzymatic Conductometric Biosensor for Detection of Heavy Metal Ions and Pesticides in Water Samples Based on Enzymatic Inhibition in Arthrospira platensis
AUTHORS:
Nadèje Tekaya, Olga Saiapina, Hatem Ben Ouada, Florence Lagarde, Philippe Namour, Hafedh Ben Ouada, Nicole Jaffrezic-Renault
KEYWORDS:
Inhibition, Phosphatase Activity, Esterase Activity, Conductometry, Arthrospira platensis
JOURNAL NAME:
Journal of Environmental Protection,
Vol.5 No.5,
April
25,
2014
ABSTRACT: An
original bi-enzymatic biosensor was designed by immobilizing Arthrospira
platensis cells, called Spirulina, on gold interdigitated transducers.
Phosphatase and esterase activities were inhibited, respectively, by heavy
metals and by pesticides. Inhibition activities were observed with different
mixtures of pesticides + heavy metals. The quantification limits for Cd2+ and Hg2+ are 10-20 M in mixture and in pure solution. The
quantification limits of parathion-methyl, paraoxon-methyl and triazine are
respectively 10-20 M, 10-18 M and 10-20 M in
mixture and pure solutions. These results show that there is no synergistic
effect between the two families of pollutants. Qualitative contamination of
effluent samples and their purification after passing through a municipal
wastewater treatment plant were observed by our bi-enzymatic biosensor and
confirmed by classical analytical techniques for heavy metal ions.