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Muller, P.Y., Janovjak, H., Miserez, A.R. and Dobbie, Z. (2002) Processing of Gene Expression Data Generated by Quantitative Real-Time RT-PCR. Biotechniques, 32, 1372-1374, 1376, 1378-1379.

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• TITLE: Involvement of GnRH and Gonadotropin Genes in Oocyte Development of Blue Gourami Females (Trichogaster trichopterus)

  AUTHORS: Gad Degani

  KEYWORDS: GnRH and Gonadotropin Genes, Blue Gourami Females

  JOURNAL NAME: Advances in Biological Chemistry, Vol.4 No.3, April 22, 2014

  ABSTRACT: The goal of this study was to gain a fundamental understanding of RnRH 1 and 3, as well as FSH and LH action, in ovarian follicles during oocyte growth by examining changes in ovarian gene transcription in blue gourami females (Trichogaster trichopterus). The levels of target ovarian mRNAs were determined by quantitative real-time PCR. GnRH1 mRNA levels were higher in oocytes at the previtellogenic stage compared to the vitellogenic and follicular oocytes maturation (FOM) stages. No significant differences in GnRH3 mRNA levels were detected among oocytes during the different stages of development. βLH and βFSH mRNA levels were lower in oocytes at the vitellogenic stage compared to the previtellogenic stage. The high transcription of GnRH 1 and 3 in the brain and of FSH and LH in the pituitary of blue gourami as was found in previous studies in our laboratory, compared to mRNA levels changed in ovary during oogenesis, supporting the hypothesis that differences exist between the function of endocrine and autocrine/paracrine of these hormones.