TITLE:
Possibility of “Comet-Like” Transport of Acetylcholine Receptors in Command Helix Neurons in Cellular Analog of Habituation
AUTHORS:
Arkady S. Pivovarov, Galina B. Murzina, Denis A. Makhnovsky, Mariya S. Tret’yakova
KEYWORDS:
CK-548, CK-636, Wiskostatin, Depression of Acetylcholine-Induced Inward Current, Command Neurons, Helix lucorum, Cellular Analog of Habituation
JOURNAL NAME:
World Journal of Neuroscience,
Vol.4 No.2,
April
22,
2014
ABSTRACT: The presence of "comet-like" radial transport of acetylcholine receptors by actin microfilaments without the participation of myosin motors in the depression of acetylcholine-induced inward chloric current (ACh-current) in command neurons of defensive behavior of the land snail, Helix lucorum, in a cellular analog of habituation was investigated. For that purpose the effects of CK548, CK-636 (inhibitors of actin-related protein complex Arp2/3, whose activation triggers rapid actin polymerization and the formation of the "comet-like" tail on the actinic filament) and wiskostatin (an N-WASP protein inhibitor, activating Arp2/3) on the depression of ACh-current were studied. The attenuation of ACh-current depression was observed upon the addition of CK548. At the same time, CK-636 and wiskostatin irreversibly strengthened the depression of this current and suppressed its spontaneous recovery. The results of CK-548 action and its mathematical modeling allow suggesting the presence of "comet-like" transport of acetylcholine receptors, initiated by Arp2/3 protein complex in receptor endo and exocytosis in command neurons of Helix lucorum in a cellular analog of habituation. Irreversible inhibition of vital metabolic processes of the neuron by wiskostatin and CK-636, which lead to the decrease in the level of ATP, could have caused irreversible effects of these blockers on current depression.