Article citationsMore>>

Shimoda, K., van Deursen, J., Sangster, M.Y., Sarawar, S.R., Carson, R.T., Tripp, R.A., Chu, C., Quelle, F.W., Nosaka, T., Vignali, D.A., Doherty, P.C., Grosveld, G., Paul, W.E. and Ihle, J.N. (1996) Lack of IL-4-Induced Th2 Response and IgE Class Switching in Mice with Disrupted Stat6 Gene. Nature, 380, 630-633. http://dx.doi.org/10.1038/380630a0

has been cited by the following article:

• TITLE: IL-1 Receptor Type II Production Is Upregulated by IL-4 and IL-13, and Downregulated by IFN-γ in Mouse Gingival Epithelial Cells

  AUTHORS: Y. Kamiya, Y. Ishihara, H. Kamei, Y. Ozawa, H. Mizutani, K. Kubo, H. Maeda, T. Noguchi

  KEYWORDS: IL-1RII, IL-4, IL-13, IFN-γ, STAT-6

  JOURNAL NAME: Modern Research in Inflammation, Vol.3 No.2, April 21, 2014

  ABSTRACT: Background and Objective: Interleukin-1 (IL-1) binds to 2 distinct and separate receptors, types I and II (IL-1RI and IL-1RII, respectively). The binding of IL-1 to IL-1RI induces cellular signaling and biological effects, whereas binding to IL-1RII does not induce cellular signaling and indirectly inhibits IL-1 biological activities such as that of the decoy receptor. Recently, Suzukiet al.reported that soluble IL-1RII (sIL-1RII) was detected in gingival crevicular fluid from a periodontitis patient. However, it remains unclear which cells produce sIL-1RII detected in periodontal tissues. We examined the localization of IL-1RII producing cells in gingival tissues as well as related production control mechanisms. Material and Methods: IL-1RII mRNA expression in gingival epithelial cells (GE1) was performed by real-time PCR analysis, while the amount of sIL-1RII production in supernatant from GE1 cells was examined by dot-blot analysis. Involvement of the phosphorylation of STAT6 in the signaling pathway was determined by western blot analysis. Statistical analysis was performed with Student’st-test. Results: Culturing with IL-4 and IL-13 significantly increased IL-1RII mRNA to levels 10.5-and 8.89-fold, respectively, above that of the control (pγ (IFN-γ) significantly suppressed IL-1RII mRNA by 0.22-fold as compared to the control (pγ, while western blotting determines the suppression of IL-1RII production by IFN-γ. Without the addition of IL-4 or IL-13 with or without IFN-γ, P-Tyr-STAT6 was not detected. Conclusion: IL-1RII mRNA expression and sIL-1RII production were increased by IL-4 and IL-13, and decreased by IFN-γ. Finally, IL-4 signaling was regulated by IFN-γ through phosphorylation of STAT6 and IL-13 signaling blockage by IFN-γ downstream of STAT6 translocation.