TITLE:
Enzyme Biosensor for Detection of Organophosphate Pesticide Residues Base on Screen Printed Carbon Electrode (SPCE)-Bovine Serum Albumin (BSA)
AUTHORS:
Ani Mulyasuryani, Mochammad Dofir
KEYWORDS:
Conductometric Biosensor; Organophosphate Pesticides; Organophosphate Hydrolase; Screen Printed Carbon Electrode
JOURNAL NAME:
Engineering,
Vol.6 No.5,
April
4,
2014
ABSTRACT: The
maximum level of organophosphate pesticide residues in rice is 0.1 mg/kg and
0.5 mg/kg in vegetables. The control of pesticide residues in agricultural
products required a method of analysis quickly and accurately. The research
developed a biosensor for the detection of organophosphate pesticide residues
in agricultural products. The research studied immobilized organophosphate
hydrolase (OPH) mass and characterization of biosensor. The solution
conductivity measurement in the conductivity cell consists of a 1 × 5 mm2 pair of electrodes screen printed carbon electrode (SPCE). The instrument is a
converted local conductometer. From the results of study concluded that the
optimum performance of the biosensor was obtained from the 105 μg OPH, at pH
8.5 with a response time of 45 seconds. In that condition the sensitivity of
biosensor is 28.04 μS/ppm and 0.18 ppm detection limit and the maximum
concentration of pesticide which can be measured is 1 ppm. Biosensors have been
applied to measure pesticide residues in some vegetable samples.