TITLE:
Genetic Diversity of Great Dane Breed Using Ten Microsatellites: Impact of Breeding Control over the Breeding Line
AUTHORS:
Gino Noris, Carla Santana, Mariana Herrán-Aguirre, Marco Antonio Meraz-Ríos, Mario Pérez-Martínez, Carlos Esquivel-Lacroix, Leonor C. Acosta-Saavedra, Eduardo Rodríguez, María de la Paz Juaréz, Emma S. Calderón-Aranda, Rocío Gómez
KEYWORDS:
Genetic Structure, Great Dane Varieties, Microsatellites, Population Genetics, Population Stratification
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.2,
April
3,
2014
ABSTRACT: The American Kennel Club and the Fédération Cynologique Internationale recognize
two phenotypic variants of Great Dane breed denominated American and European
varieties. Historically, these varieties have been segregated according to morphological
characteristics. In an attempt to obtain a better characterization, breeders have
been interested in the genetic parameters that could evaluate the within-breed diversity.
In this document, we studied the genetic structure of Great Dane breed with 10
STR markers in 88 dogs using capillary electrophoresis. Cluster analysis,
population differentiation and phylogenetic analyses revealed that American and
European varieties are genetically independent. Nevertheless, within the
American variety a genetic stratification was found. Additionally, a high misclassification
(28%) was detected, which could be due to wrong registration or false
paternity. Our results support the importance to deem genetic markers as useful
tools in breeding control. Similarly, these studies serve as an accurate reference
to establish standards by dog breeding associations and for choosing among dogs
for inter-breeding. Nevertheless, genetic tools are only a complement of
morphological methods, since both are reshuffling the control over the breeding
line. Notwithstanding, this database provides an overall and scape concerning the
impact of genetic diversity within-breed. Unmistakably, more databases are
needed to increase the quality of the breeding line as well as the number of
STR in order to study, with more detail, the genetic structure in the Great
Dane race.