TITLE:
Obtaining Bacteriocins by Chromatographic Methods
AUTHORS:
Valery Borzenkov, Vladimir Surovtsev, Ivan Dyatlov
KEYWORDS:
Purification; Bacteriocins; Surface Cell Fragments; Hydrophobicity; Membranes; Ion-Exchange; Hydrophobic Chromatography
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.5,
April
2,
2014
ABSTRACT: Bacteriocins are a large group of chromosome or plasmid-encoded and ribosomally synthesized low-molecular-weight (2 to
6 kDa) antimicrobial and amphiphilous peptides produced by Gr+ or Gr- bacteria [1]. Their low toxicity as well as the absence of
allergenicity and reactogenicity is confirmed by testing selected bacteriocins [2] [3]. Bacteriocins can be widely used as
preservatives and antibiotic alternatives in medicine. Nisin, a Streptococcus lactis-derived
bacteriocin, has been in practice in food industry for a long time. A relevant
product contains about 2.5% of nisin. For medical use (e.g., when injected into
the blood stream), highly purified drugs are required. However, the yield of
bacteriocins accounts for no more than a few percents from the total activity
in the culture liquid. In this paper, we propose methods (by example of two B. subtilis strains), allowing to increase the yield up to ~80%. It is believed that other
bacteriocins may be purified by these methods and with the same yield.