TITLE:
Evaluation of Phenolic Content and Antioxidant Property of Crossandra infundibuliformis Leaves Extracts
AUTHORS:
Kumar G. Patil, V. Jaishree, H. N. Poornachandra Tejaswi
KEYWORDS:
Crossandra infundibuliformis; DPPH; Hydrogen Peroxide; Nitric Oxide; Ascorbic Acid; Gallic Acid
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.9,
April
1,
2014
ABSTRACT: The present study was aimed to investigate the phenolic content and
antioxidant property of Crossandra infundibuliformis leaves. Shade dried leaves were successively extracted and determined phenolic
and flavonol content of the extracts. Six different in vitro antioxidant assays such as 1, 1’-diphenyl-2-picrylhydrazyl (DPPH), lipid peroxidation (LPO), nitric oxide,
hydrogen peroxide radical scavenging, reducing power of the extracts and total
antioxidant capacity (values were expressed as mg ascorbic acid
(vitamin C) equivalent antioxidant capacity per gram of extracts) assays were
followed to determine the antioxidant capacity of the extracts. The results of
this study indicated that methanol extract possess higher level of phenolic
(98.52 mg of gallic acid/g of
extract) and flavonol (84.59
mg of rutin/g of extract)
constituents. The antioxidant property of ethyl acetate extract was
significantly higher than other extracts in DPPH method. Moreover, methanol
extract has significantly higher antioxidant activity in LPO inhibition (IC50 value of 70.66 μg/ml), hydrogen peroxide (IC50 value 130.33 μg/ml)
and reducing capacity of the extract (1.19) than other chloroform and ethyl
acetate extracts. The strong correlation was observed for phenolic content and
antioxidant activities of the extracts ensuring the involvement of phenols in
antioxidant activity. However, results suggests that Crossandra infundibuliformis leaves possess antioxidant activity.