TITLE:
Analysis of Rice Grain Quality-Associated Quantitative Trait Loci by Using Genetic Mapping
AUTHORS:
Byung-Wook Yun, Min-Gyu Kim, Tri Handoyo, Kyung-Min Kim
KEYWORDS:
Grain Quality; QTL; Rice; Genetic Map; Doubled Haploid
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.9,
April
1,
2014
ABSTRACT: The main objective of this research was to identify quantitative trait
loci associated with rice qualities to provide reliable information for
marker-assisted selection and development of new varieties. In total, 120
doubled haploid (DH) lines developed by another culture from the F1 hybrid of a cross between “Cheongcheong”, a Tongil variety, and “Nagdong”, a
japonica variety, were used. A microsatellite linkage map of 222 markers
spanned 2082.4 centimorgans (cM) and covered 12 rice chromosomes with an
average interval of 9.4cM between markers. Eight quantitative trait loci (QTLs)
were associated with rice quality, consisting of two QTLs on chromosomes 1 and
9 for amylose content; three QTLs on chromosomes 8, 9, and 10 for protein
content; and three QTLs on chromosomes 2, 3, and 6 for lipid content. PCR
expression levels measured using the SSR markers RM23914 for proteins and
RM6266 for lipids, and RM586 showed a higher degree of amplification. The
present study should be useful for improving the nutritional quality of rice by
means of marker-assisted selection.