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Booth, N.L., Overk, C.R., Yao, P., Totura, S., Deng, Y., Hedayat, A.S., Bolton, J.L., Pauli, G.F. and Farnsworth, N.R. (2006) Seasonal Variation of Red Clover (Trifolium pratense, L. fabaceae) Isoflavones and Estrogenic Activity. Journal of Agricultural and Food Chemistry, 54, 1277-1282.
http://dx.doi.org/10.1021/jf052927u

has been cited by the following article:

• TITLE: Secondary Metabolites in Flax Root Extracts at Various Stages of Maturity and Effects on Proliferation and Cytotoxicity in Oestrogen-Receptor-Positive Breast Cancer Cells

  AUTHORS: Marlen Szewczyk, Nicole Strater, André Schlichting, Volker Briese, Dagmar-Ulrike Richter

  KEYWORDS: Flax Root; Stage of Maturity; Lignans; Breast Cancer; MCF7

  JOURNAL NAME: Advances in Biological Chemistry, Vol.4 No.2, March 31, 2014

  ABSTRACT: Flax contains large amounts of hormone-like compounds, especially lignans. These socalled phytoestrogens are thought to inhibit the cell growth of hormone-sensitive cancers. Hence, we analysed the influence of flax root extracts at various stages of maturity on the proliferation and cytotoxicity in oestrogen-receptor-positive breast cancer cells (MCF7) in vitro. Flax root extracts were prepared by using lignan extraction. The extracted compounds were analysed by Pyrolysis-Field Ionisation Mass Spectrometry. Various extract concentrations were applied to the cells to test for proliferation (BrdU test) and cytotoxicity (LDH test). A significantly higher inhibition of cell proliferation was observed with an extract made from 9-week-old flax roots in comparison with that of 3- and 6-week-old roots. Older roots contained more lignans and other phenolic substances than younger roots. The maturity grade of plants or their various parts is thus important for the production and concentration of secondary metabolites and leads to different biological effects on breast cancer cell growth.