TITLE:
Thermal Water from Uriage-les-Bains Exerts DNA Protection, Induction of Catalase Activity and Claudin-6 Expression on UV Irradiated Human Skin in Addition to Its Own Antioxidant Properties
AUTHORS:
Francine Joly, Jean-Eric Branka, Luc Lefeuvre
KEYWORDS:
Human Skin; UVA-UVB; Thermal Water from Uriage-les-Bains; Claudin-6; Antioxidant Effect; SOD-Like Effect; Catalase
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.4 No.2,
March
28,
2014
ABSTRACT: Aim: In order to decipher the mechanisms
underlying the known protective effects of the thermal water from
Uriage-les-Bains (TWFULB) on the skin barrier function, we studied its antioxidant
properties as well as its effect on the expression of the tight-junctional
protein claudin-6, a candidate tumor suppressor factor. Study Design/Methods: In a
first step, TBARS and SOD activity assays were performed in an in vitro model of human dermal fibroblasts
treated by hypoxanthine/xanthine oxidase (HO/XO) mixture, in order to evaluate
the own antioxidant effect of the thermal water. In a second step, human
keratinocytes irradiated or not by UVB were used to evaluate the protective
role of TWFULB on nuclear DNA damage using a comet assay. In a third step, an ex vivo model of human skin explants
irradiated or not by UVA and UVB was used to evaluate the effect of TWFULB on
the intracellular catalase activity and on the cutaneous claudin-6 expression. Results: TWFULB showed
significant protective effects against oxidative stress induced by HO/XO: the
cell viability was improved and the lipid peroxidation was reduced. The tested
thermal water also showed significant SOD-like activity and protective effect
on the UVB-stressed DNA. Considering the ex
vivo models of skin explants,
TWFULB was able to counterbalance the “negative” effect of UVB on the intracellular
catalase activity and on the cutaneous claudin-6 expression.Conclusion:
This multiparametric approach shows the antioxidant
activity of TWFULB and emphasizes its role in the DNA protection of the
cutaneous tissue in front of the UV irradiations, and finally suggests that
some effects could involve the candidate suppressor functions of claudin-6.