TITLE:
A Rapid, Validated RP-HPLC Method for the Determination of Seven Volatile N-Nitrosamines in Meat
AUTHORS:
Mohammad Al-Kaseem, Zaid Al-Assaf, François Karabeet
KEYWORDS:
N-Nitrosamines; Rp-HPLC; Food Safety
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.5 No.3,
March
27,
2014
ABSTRACT: The present work reports a rapid
reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination
of seven volatile N-nitrosamines namely N-nitrosodimethylamine (NDMA),
N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosodiproylamine (NDPA),
N-nitrosodi butylamine (NDBA), N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine
(NPYR) for monitoring food safety. A strategic experimental approach was
implemented for the method development. The desired chromatographic separation
was achieved on a Symmetry C18 (4.6 × 150 mm, 5 μm) column using gradient
elution. The optimized mobile phase consisted of the 10 mM ammonium hydroxide
pH = 8.9 and acetonitrile. The
eluted compounds
were monitored at 231 nm wavelength using spectrophotometric detector. The
developed method separated seven compounds from each other within a run time of
10 min. The method is effective for the determination of presence of these
carcinogenic compounds. The average extraction recovery of seven
nitrosamines was found 84.5%; the precision of method was found less than 2.7% and accuracy was found between
95%-102.5%. The assay could be
applied in food monitoring safety.