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Bellec, G.F., Cauvin, M.C., Calve, K.L., Dreano, Y., Gouerou, H., Menez, J.F. and Berthou, F. (1996) Analysis of N-Nitrosamines by High-Performance Liquid Chromatography with Post-Column Photohydrolysis and Colorimetric Detection. Journal of Chromatography A, 727, 83-92. http://dx.doi.org/10.1016/0021-9673(95)01073-4

has been cited by the following article:

• TITLE: A Rapid, Validated RP-HPLC Method for the Determination of Seven Volatile N-Nitrosamines in Meat

  AUTHORS: Mohammad Al-Kaseem, Zaid Al-Assaf, François Karabeet

  KEYWORDS: N-Nitrosamines; Rp-HPLC; Food Safety

  JOURNAL NAME: Pharmacology & Pharmacy, Vol.5 No.3, March 27, 2014

  ABSTRACT: The present work reports a rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of seven volatile N-nitrosamines namely N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosodiproylamine (NDPA), N-nitrosodi butylamine (NDBA), N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine (NPYR) for monitoring food safety. A strategic experimental approach was implemented for the method development. The desired chromatographic separation was achieved on a Symmetry C18 (4.6 × 150 mm, 5 μm) column using gradient elution. The optimized mobile phase consisted of the 10 mM ammonium hydroxide pH = 8.9 and acetonitrile. The eluted compounds were monitored at 231 nm wavelength using spectrophotometric detector. The developed method separated seven compounds from each other within a run time of 10 min. The method is effective for the determination of presence of these carcinogenic compounds. The average extraction recovery of seven nitrosamines was found 84.5%; the precision of method was found less than 2.7% and accuracy was found between 95%-102.5%. The assay could be applied in food monitoring safety.