TITLE:
Evaluation of the Immunoregulatory Capacities of Feed Microbial Materials in Porcine Intestinal Immune and Epithelial Cells
AUTHORS:
Naosuke Kumagae, Julio Villena, Yohsuke Tomosada, Hisakazu Kobayashi, Paulraj Kanmani, Hisashi Aso, Takashi Sasaki, Motohiko Yoshida, Hiroshi Tanabe, Isao Shibata, Tadao Saito, Haruki Kitazawa
KEYWORDS:
Feed Microbial Material; Immunoregulatory Effect; Porcine Intestinal Epitheliocytes; Porcine Immune Cells
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.4 No.3,
March
21,
2014
ABSTRACT: The establishment
of drug-free feeding systems has been required for secure and healthy livestock
production. Although functional feed materials containing microorganisms as
alternatives to enhance intestinal immunity are expected to be beneficial for
reducing diarrhoea caused by pathogens in weaned piglets, the effects of such
materials on porcine intestinal cells have not been investigated in detail.
Therefore, this work evaluated the immunoregulatory functions of microbial feed
materials in porcine intestinal immune and epithelial cells. Porcine immune
cells isolated from Peyer’s patches and mesenteric lymph nodes were stimulated
with six different feed materials containing microorganisms, and evaluated for
lymphocyte mitogenicity and cytokine inductions. In addition, porcine
intestinal epithelial cells were stimulated with the materials before treatment
with heat-killed enterotoxigenic Escherichia
coli (ETEC), and analyzed for the proinflammatory cytokine expressions. The
material containing Bifidobacterium
thermophilum significantly augmented lymphocytes’ mitogenicity and also
induced a high expression of IL-2, IL-6 and IFN-γ in immune cells, and inhibited ETEC-induced overexpression of
IL-6 and IL-8 via regulation of Toll-like receptor signaling. These results
suggest that this feed material stimulates intestinal epithelial and immune
cells to exert immunoregulation, suggesting that this feed is expected to
contribute to promoting the health of piglets without using antimicrobial feed
materials.