TITLE:
Combination of Acacetin with Antibiotics against Methicillin Resistant Staphylococcus aureus Isolated from Clinical Specimens
AUTHORS:
Jeong-Dan Cha, Sung-Mi Choi, Jeong Hye Park
KEYWORDS:
Acacetin; Methicillin-Resistant Staphylococcus aureus; Minimum Inhibitory Concentrations; Minimum Bactericidal Concentrations; Time-Kill Curves; Fractional Inhibitory Concentration
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.4,
March
20,
2014
ABSTRACT: Methicillin-restitant Staphylococcus aureus (MRSA) is very dangerous bacteria and one of
the most feared nosocomial germs. In this study, acacetin was evaluated against
20 clinical isolates of MRSA, either alone or in combination with antibiotics.
The acacetin exhibited a good activity against isolates MRSA with MICs/MBCs
ranged between 10-80/20-160 μg/mL, for ampicillin 64-1024/128-2048 μg/mL, and for oxacillin 8-32/16-64 μg/mL. The combination of acacetin plus
oxacillin or ampicillin was reduced by ≥4-fold against isolates MRSA tested, evidencing a
synergistic effect as defined by a FICI of ≤0.5. Furthermore, a
time-kill study evaluating the growth of the tested bacteria was completely
attenuated after 2-5 h of treatment with the 1/2 MIC of acacetin,
regardless of whether it was administered alone or with oxacillin (1/2 MIC) or
ampicillin (1/2 MIC). In conclusion, acacetin exerted synergistic effects when
administered with oxacillin or ampicillin and the antibacterial activity and
resistant regulation of acacetin against clinical isolates of MRSA might
be useful in controlling MRSA infections.