TITLE:
New Technique to Create a Suspension Containing Bacteriophages and How It Can Be Used to Control Cabbage Leaf Spot Caused by Xanthomonas campestris PV. campestris
AUTHORS:
Igor Villela Marroni, José Carlos Germani
KEYWORDS:
Biocontrol; Virus; Bacteria; Disease Index
JOURNAL NAME:
Agricultural Sciences,
Vol.5 No.4,
March
20,
2014
ABSTRACT: The purpose of the present study was to develop a new
method for producing and identifying a bacteriophage suspension and research
its potential against plant bacteria. The suspension obtained with the new
technique was effective in controlling Xanthomonas
campestris PV. campestris both in vitro and in vivo. A difference was observed between the suspension with
pathogen (PLP) and crude suspension with lesioned plant without the pathogen
originated from it (PLN). Clear zones were observed at 48 h/28°C in both (PLS)
suspension added saccharose and (PLP). Although PLP and PLS treatments showed
no statistical difference in CFU values (5 × 105 and 1 × 105, respectively),
they differed from the crude suspension (PLN) and the control. Then, in vivo assessment of suspensions with
clear zones in the dishes was performed. In
vivo (PLPS) and (PLPP) treatments consisting of inoculum were added to the
crude suspensions (PLS and PLP) to increase bacteriophages. A decrease in the
disease severity index compared to the control was observed, showing that
treatment with the phage suspension protects the plant. The lowest disease
indexes were reported with (PLP) and (PLS), with values of 1.46 and 1.40,
respectively. The highest averages in dry matter (5.1 inboth treatments) were
obtained with (PLP) and (PLS) treatments.