TITLE:
Highly Efficient in Vitro Regeneration Method of Endangered Medicinal Plant Heliotropium kotschyi (Ramram) in the Kingdom of Bahrain
AUTHORS:
Manal Ahmed Sadeq, Malabika Roy Pathak, Ahmed Ali Salih, Mohammed Abido, Asma Abahussain
KEYWORDS:
Ex Situ Conservation; Heliotropium kotschyi; Organogenesis; Plant Regeneration
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.5,
March
19,
2014
ABSTRACT: Heliotropium kotschyi (Ramram),
is a very important plant in
relation to environment protection and for its medicinal value in Bahrain. It
has been detected as one of the endangered plants in Bahrain. Ex
situ conservation of this plant
applying tissue culture method was used in the present study. In vitro plant regeneration has been established
via shoot organogenesis using nodal meristems. The effect of various plant
growth regulators was evaluated to regenerate the whole plants by in vitro micropropagation in
modified MS media supplemented with different plant growth regulators (PGRs). In
vitro regeneration of plants was involved in three steps by shoot initiation,
multiplication and rooting of microshoots. Initially 100% explants of Heliotropium
kotschyi responded to initiate shoot in MS medium supplemented with 8.88 μM
BAP with 5.71 μM IAA after 4 weeks of culture. Multiplication of shoots and
ultimate plant regeneration capacity of Heliotropium kotschyi were
compared and the highest performance of 578 shoots/explant was observed in
presence of 8.88 μM BAP and 5.71 μM IAA after second transfer. Rooted plantlets
were transferred to soil pots and 60% plants were survived after one and a half
months.
The highly efficient plant regeneration protocol of the desert plant is of great value in rapid plant propagation program for the purpose of
conservation of biodiversity.