TITLE:
In Vitro Regeneration of Curcuma caesia Plantlets from Basal Part and via Somatic Embryogenesis
AUTHORS:
Ab. Rahman Zuraida, Kamaruddin Fatin Liyana Izzati, Othman Ayu Nazreena, Che Mohd Zain Che Radziah, Sheikh Ghadzi Siti Nur Asyikin, Subramaniam Sreeramanan
KEYWORDS:
In Vitro; Regeneration; Somatic Embryogenesis; Curcuma caesia
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.4,
March
12,
2014
ABSTRACT: Plantlets of Curcuma caesia were produced in
vitro from newly sprouting vegetative buds of tubers. Segments of the
plantlets from the junction between the root and the basal portion of the stem
were subsequently used as explants to investigate factors affecting callus
induction and plant regeneration via somatic
embryogenesis. The explants were placed on Woody Plant Medium (WPM) together
with different concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and
benzyl aminopurine (BAP) in the presence of light. The growth medium
supplemented with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after
70 days of culture. Sub-culturing on the same medium enhanced the production of
friable callus. Culture media containing higher concentrations of agar promoted
the development of green somatic embryos from the callus. Respond of somatic
embryogenesis was most successful with MS medium in 6.0 g/L agar supplemented
with 5 mg/L BAP and 0.2 mg/L 2,4-D whereby the callus developed into green
somatic embryos with an efficiency of 53%. This culture medium also produced
the largest number plantlets.