TITLE:
Rapid, Efficient and High-Performance Protocol for Agrobacterium rhizogenes-Mediated Hairy Root Transformation of the Common Bean Phaseolus vulgaris
AUTHORS:
Sanghamitra Khandual, Pallavolu Maheswara Reddy
KEYWORDS:
Phaseolus vulgaris; Agrobacterium rhizogenes; Hairy Root Transformation; GUS Expression
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.4,
March
12,
2014
ABSTRACT: A rapid, efficient and high-performance transformation
protocol employing Agrobacterium rhizogenes was developed for the common bean Phaseolus vulgaris. In
this study, we examined competencies of various protocols to induce and
explants that respond to hairy root transformation in bean plants. Utilizing
young seedlings with severed radicles/hypocotyls, we developed a highly efficient
procedure for achieving hairy root transformation frequencies as high as 100%
as visualized by GUS reporter gene expression system. Transgenic hairy roots in
these young composite plants were
susceptible to nodulation by rhizobia, and form an excellent system for high
throughput genomic analysis to study root biology and endosymbiosis in
common bean.