TITLE:
Sex Difference in Lactate Production by Adipocytes from Lean Humans
AUTHORS:
Danilo Roberto X. de O. Crege, Henrique José V. Silveira, Élinton Adami Chaim, José Carlos Pareja, Alain Géloen, Dora Maria Grassi-Kassisse
KEYWORDS:
Adipose Tissue; Glycerol; Humans; Lactate; Lipolysis
JOURNAL NAME:
Open Journal of Endocrine and Metabolic Diseases,
Vol.4 No.3,
March
11,
2014
ABSTRACT: Lactate is an anaerobic
metabolite produced in the absence of an adequate O2 supply.
Although for a long time considered to be a waste product of glycolysis
resulting from hypoxia, lactate is, in fact, an important source of glucose and
also a gluconeogenic precursor, having a role in metabolic and endocrine
signaling. Lactate is produced by adipocytes and muscle cells. Objectives: In this study, we investigated the sex
differences in lactate production by adipocytes isolated from lean human
visceral adipose tissue. Main Methods: The experiments described were done
using adipocytes isolated from adipose
tissue of lean men and women. Adipocytes were isolated following Rodbell
procedure, with modifications, for posterior analysis of glycerol and lactate
production. Results: Morphometric analysis revealed no significant differences
in the size of adipocytes from men and women (diameter: men: 172 ± 24 μm vs. women: 160 ± 16 μm, n = 4 and
10, respectively). Basal glycerol production was significantly higher in
adipocytes from men compared to women (0.34
± 0.06 vs. 0.16 ± 0.01 μmol/106 cells/60
min; mean ± SEM, n = 7 and 4, respectively; p