TITLE:
Development of a Rapid and Efficient Liquid Chromatography Method for Determination of Gibberellin A4 in Plant Tissue, with Solid Phase Extraction for Purification and Quantification
AUTHORS:
Julia Medrano Macías, Rahim Foroughbakhch Pournavab, Manuel Humberto Reyes-Valdés, Adalberto Benavides-Mendoza
KEYWORDS:
Dasyrilon cedrosanum; Epithelantha micromeris; Phytohormone Separation; Plant Growth Regulators; Reverse Phase Liquid Chromatography; Solid Phase Extraction
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.5,
March
11,
2014
ABSTRACT: A new, rapid and efficient reverse phase
Liquid Chromatography (RP-LC) method was developed for determination of
Gibberellin A4 (GA4) in samples of flower stalk of Dasylirion cedrosanum and
vegetative tissue of Epithelantha
micromeris. Purification of GA4 was carried out by solid phase extraction
(SPE), in Epithelantha micromeris. In
the chromatography method was obtaining a retention time of 2.1 min, using
Hypersil GOLD C-18 column (100 × 4.6 mm dim and size particle 5 μ), mobile
phase 50/50 acetonitrile/water and a flow 1.0 ml/min. Detection was carried out
by a UV detector set at 205 nm, and a quantization limit of 0.4 mg/L. The
obtained correlation coefficient was 0.995.