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Clinical Laboratory Standards Institute (CLSI) (2006) Performance Standards for Antimicrobial Susceptibility Testing. Sixth Informational Supplement CLSI Document M100-516, Wayne.

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• TITLE: Occurrence of Escherichia coli O157 in Retailed-Beef and Related Meat Products in Zaria, Nigeria

  AUTHORS: Salome Y. Tafida, Jacob K. P. Kwaga, Mohammed Bello, Junaidu Kabir, Veronica J. Umoh, Sabo E. Yakubu, Andrew J. Nok

  KEYWORDS: E. coli O157; Meat Products; Multiple Drug Resistance; Food Safety

  JOURNAL NAME: Food and Nutrition Sciences, Vol.5 No.6, March 7, 2014

  ABSTRACT: This study evaluated retailed-beef and related meat products for E. coli O157 in Zaria, Nigeria. Sample types included raw meat, “suya” (roasted meat), “balangu” (barbequed meat), “kilishi” (spiced sun dried meat) and “dambu” (shredded fried meat). A total of 182 samples were analyzed for E. coli O157. Isolates were characterized using conventional biochemical methods and Microbact 12E test kit. Susceptibilities of the isolates to 18 commonly used antimicrobial agents were determined by the disk diffusion method. The carriage of stx1 and stx2 genes was determined by PCR. Microbact confirmed 4 E. coli isolates. All isolates exhibited multiple drug resistance to the antimicrobial agents tested. An overall prevalence of 2.2% was obtained for E. coli O157. All 4 isolates of E. coli O157 were isolated from raw meat; two of which harboured the stx1 gene.