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Parasharami, V.A., Naik, V.B., von Arnold, S., Nadgauda, R.S. and Clapham, D.H. (2006) Stable Transformation of Mature Zygotic Embryos and Regeneration of Transgenic Plants of Chir Pine (Pinus roxburghii Sarg). Plant Cell Reports, 24, 708-714. http://dx.doi.org/10.1007/s00299-005-0019-z

has been cited by the following article:

• TITLE: Development of a Genetic Transformation Method for Seabuckthorn (Hippophae rhamnoides L.)

  AUTHORS: Sridevy Sriskandarajah, David Clapham, Per-Olof Lundquist

  KEYWORDS: Hippophae rhamnoides; Particle Bombardment; Transformation

  JOURNAL NAME: American Journal of Plant Sciences, Vol.5 No.5, March 7, 2014

  ABSTRACT: Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing high amounts of several bioactive compounds with nutritional and medicinal traits. It is also planted to control soil erosion. A genetic transformation procedure will facilitate studies of the control of plant development and interactions with symbionts and pathogens, and will provide a tool for plant breeding. Here, we present a particle bombardment method for transforming seabuckthorn. The early stages of induced adventitious shoots from roots were chosen as a novel target tissue for the transformation procedure. The root system was bombarded with gold particles coated with plasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression, and shoots were regenerated under kanamycin selection. PCR analysis of the regenerated transformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kb fragment from the 35S promoter in three shoots from independent transformation events.