Article citationsMore>>

Clapham, D., Elfstrand, M., Sabala, I., Von Arnold, S., Demel, P. and Koop, H.-U. (2000) Gene Transfer by Particle Bombardment to Embryogenic Cultures of Picea abies and the Production of Transgenic Plantlets. Scandinavian Journal of Forest Research, 15, 151-160. http://dx.doi.org/10.1080/028275800750014957

has been cited by the following article:

• TITLE: Development of a Genetic Transformation Method for Seabuckthorn (Hippophae rhamnoides L.)

  AUTHORS: Sridevy Sriskandarajah, David Clapham, Per-Olof Lundquist

  KEYWORDS: Hippophae rhamnoides; Particle Bombardment; Transformation

  JOURNAL NAME: American Journal of Plant Sciences, Vol.5 No.5, March 7, 2014

  ABSTRACT: Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing high amounts of several bioactive compounds with nutritional and medicinal traits. It is also planted to control soil erosion. A genetic transformation procedure will facilitate studies of the control of plant development and interactions with symbionts and pathogens, and will provide a tool for plant breeding. Here, we present a particle bombardment method for transforming seabuckthorn. The early stages of induced adventitious shoots from roots were chosen as a novel target tissue for the transformation procedure. The root system was bombarded with gold particles coated with plasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression, and shoots were regenerated under kanamycin selection. PCR analysis of the regenerated transformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kb fragment from the 35S promoter in three shoots from independent transformation events.