TITLE:
Preparation of Microcapsules Containing Aqueous Solution of Azur B with Melting Dispersion Cooling Method and Application to DNA Amplification Detector
AUTHORS:
Yoshinari Taguchi, Ryohei Yamamoto, Natsukaze Saito, Masato Tanaka
KEYWORDS:
Microcapsules; Azur B; DNA Amplification Detector; Melting Dispersion Cooling Method; Multiple Emulsion
JOURNAL NAME:
Journal of Encapsulation and Adsorption Sciences,
Vol.4 No.1,
March
5,
2014
ABSTRACT: Microcapsules
containing the aqueous solution of Azur B of a water soluble dye were prepared
with the melting dispersion cooling method and applied to the amplification detector
of plant DNA. Paraffin wax with melting temperature of 75°C was used as the
shell material. In
the experiment, the aqueous solution (W) of Azur B as the core material was
dispersed in the melted paraffin wax (O) to form the (W/O) emulsion and then,
the (W/O) emulsion was dispersed in the silicon oil (O’) as the continuous
phase to form the (W/O)/O’ emulsion at 85°C. After formation of the (W/O)/O’
emulsion, the microcapsules were prepared by cooling the (W/O)/O’ emulsion to
50°C. The microcapsules
were prepared by changing the concentration of oil soluble surfactant in the
(W/O) emulsion and the volume of the (W/O) emulsion in the (W/O)/O’ emulsion. The
microencapsulation efficiency increased with the concentration of oil soluble
surfactant and finally became 100% under the optimum conditions. Furthermore, the
microcapsules were melted down at temperature of 85°C to reveal the sharp
thermal responsibility and to release the aqueous solution of Azur B. As a result, it was
found that the microcapsules were able to be applied to the amplification
detector of plant DNA by utilizing the reaction between DNA and Azur B.