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de Beer, J.L., van Ingen, J., de Vries, G., Erkens, C., Sebek, M., Mulder, A., Sloot, R., van den Brandt, A.M., Enaimi, M., Kremer, K., et al. (2013) Comparative Study of IS6110 Restriction Fragment Length Polymorphism and VariableNumber Tandem-Repeat Typing of Mycobacterium Tuberculosis Isolates in the Netherlands, Based on a 5-Year Nationwide Survey. Journal of Clinical Microbiology, 51, 1193-1198. http://dx.doi.org/10.1128/JCM.03061-12

has been cited by the following article:

• TITLE: GenoType MTBDRplus as a Complementary Tool for the Typing of Mycobacterium tuberculosis

  AUTHORS: Timothée Ouassa, Guillaume Yao Loukou, Hortense Faye-Kette

  KEYWORDS: Mycobacterium tuberculosis; MIRU-VNTR; Spoligotyping; GenoType MTBDRplus; Côte d’Ivoire

  JOURNAL NAME: Advances in Infectious Diseases, Vol.4 No.1, March 4, 2014

  ABSTRACT: The aim of this study was to investigate the usefulness of combining profiles obtained by using a line probe assay (LPA) originally intended to characterize the resistance of two major anti-tuberculosis drugs to the association of spoligotyping and MIRU-VNTR, in order to improve its discriminatory power. For this purpose, 74 strains of Mycobacterium tuberculosis belonging to the same cluster after spoligotyping were further typed by using the 24 loci MIRU/VNTR. These strains were then tested by the GenoType MTBDRplus, and profiles obtained were analyzed within previously obtained clusters. The combination of spoligotying and MIRU-VNTR led to the consolidation of 56 of them (75.7%) in 9 clusters. Most of the strains (54, 96.4%) were multidrug resistant (MDR). From the 9 initial clusters, the addition of GenoType MTBDRplus helped to define 26 profiles including 11 unique profiles, and 3 original clusters remained undifferentiated. Results obtained express the relevance of combining this method which improved quite significantly the discriminatory power in typing Mycobacterium tuberculosis.