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Deed, R., Rooney, P., Kumar, P., Norton, J.D., Smith, L., Freemont A.J. and Kumar, S. (1997) Early-Response Gene Signalling Is Induced by Angiogenic Oligosaccharides of Hyaluronan in Endothelial Cells. Inhibition by Non-Angiogenic, High-Molecular-Weight Hyaluronan. International Journal of Cancer, 71, 251-256.
http://dx.doi.org/10.1002/(SICI)1097-0215(19970410)71:2<251::AID-IJC21>3.0.CO;2-J

has been cited by the following article:

• TITLE: In Vitro Analysis of VEGF and HGF Production by Fibroblast in Cultured Dermal Substitute Combined with EGF-Incorporating Top Dressing

  AUTHORS: Emi Iijima, Daichi Daichi Toyoda, Akiko Yamamoto, Misato Kuroyanagi, Yoshimitsu Yoshimitsu Kuroyanagi

  KEYWORDS: Cultured Dermal Substitute; Fibroblast; Collagen; Hyaluronic Acid; Epidermal Growth Factor

  JOURNAL NAME: Open Journal of Regenerative Medicine, Vol.3 No.1, March 3, 2014

  ABSTRACT: This study aimed to investigate the potential of cultured dermal substitute (CDS) to release angiogenic growth factors when laminated with a membrane containing epidermal growth factor (EGF) as a top dressing. Membranes were prepared by air-drying a solution of hyaluronic acid (HA) and collagen (Col) with or without EGF. Membranes were designed to contain EGF at concentrations of 0, 0.1, 0.2 or 0.5 μg/cm2. CDS was prepared by incorporating fibroblasts into a collagen gel combined with a cross-linked HA spongy matrix, followed by culturing for 5 days. CDS was designed to contain fibroblasts at a density of 2 × 105 (Group I) or 4 × 105 cells/cm2> (Group II). CDS was elevated at the interface between air and culture medium, on the top of which each membrane was placed. This culture system was employed as a wound surface model. Metabolic activity of the fibroblasts in the CDS cultured for 7 days on a wound surface model was measured by MTT assay. The amounts of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) after 7 days of cultivation were measured by using ELISA. Membranes containing EGF ranging from 0.1 to 0.5 μg/cm2> facilitated production of both VEGF and HGF, as compared with control membranes without EGF. However, a membrane containing EGF at a concentration of 0.5 μg/cm2> failed to facilitate fibroblast cytokine production in Group I. These results demonstrated that the EGF-incorporating membrane was able to stimulate fibroblasts in the CDS to synthesize an increased amount of VEGF and HGF in a dose-dependent manner.