TITLE:
Protective Effect of Camellia sinensis on Methotrexate-Induced Small Intestinal Mucositis in Mice
AUTHORS:
Samuel Brito de Almeida, Manuel Carlos Serra Azul Monteiro, Ana Valêska Pinto de Lima, Dalgimar Beserra de Menezes, Sandra Maria Nunes Monteiro
KEYWORDS:
Mucositis; Vitamin E; Camellia Sinensis; Morphometry; Mice; Cathechins
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.5 No.5,
February
26,
2014
ABSTRACT:
Background: Green tea has been used as a daily beverage for several
years. Anti-inflammatory effect of tea has also been depicted in different
papers. Therefore we had set forward this study to examine the potential anti- inflammatory activity of green tea in small intestine
mucositis experimental models. Aims: Evaluation of anti- inflammatory effects of green tea on mice. Materials and
Methods: Green tea decoction (20%) was prepared by soaking 20 g of green tea in 100 ml
boiled water separately, soaked for 2 minutes and thereafter filtered. In- flammatory activity was induced using methotrexate (2.75 g/kg/24 h sc), and a protecting effect of
mucositis con- dition was investigated by vitamin E and Camellia sinensis decoction. Study
Design: An experimental study was approved by an Animal Ethical Commitee. Results: Green tea decoction (20%) has shown
significant anti-in- flammatory effects (65%
and 70%) on methotrexate-induced acute mucositis model. In villous atrophy
Green tea decoction (10% and 20%) has shown no protecting action at different
intestinal segments. But at intestinal crypt hyperplasia, green tea decoction
has shown 65.74%, as compared to mucositis group. An increase of apoptotic
bodies were acchieved at MTX group, CS reduced this occurrence. Conclusion:
Taken together, our data indi- cate that
green tea (20%) has a potential anti-inflammatory compared vitamin E
antioxidant action and cor- roborates with
the current trend of tea being promoted as “health drink”. However more
pharmacological and biochemical assays is necessary to elucidate mechanisms.