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C. Callens, S. Coulon, J. Naudin, I. Radford-Weiss, E. Raffoux, P. H. Wang, S. Agarwal, H. Tamouza, E. Paubelle, V. Asnafi, J. A. Ribeil, P. Dessen, D. Canioni, O. Chandesris, M. T. Rubio, C. Beaumont, M. Benhamou, H. Dombret, E. Macintyre, R. C. Monteiro, I. C. Moura and O. Hermine, “Targeting Iron Homeostasis Induces Cellular Differentiation and Synergizes with Differentiating Agents in Acute Myeloid Leukemia,” The Journal of Experimental Medicine, Vol. 207, No. 4, April 2010, pp. 731-750. doi:10.1084/j

has been cited by the following article:

• TITLE: The Effect of Hemin and Deferoxamine on Selenium, Zinc, and Iron Levels of K562 Cells

  AUTHORS: Bahire Küçükkaya, Lale Afrasyap

  KEYWORDS: Hemin, Deferoxamine, DFO, K562, Selenium, Zinc, Iron, Trace Elements

  JOURNAL NAME: Journal of Cancer Therapy, Vol.2 No.1, March 15, 2011

  ABSTRACT: This study investigated contents of trace elements selenium, zinc and iron both in control K562 cells, human leukemia cell line, and cells treated with hemin or the iron chelator deferoxamine cells. K562 cell line was grown in RPMI medium supplemented with 10% fetal calf serum, 100 IU/mL penicillin, 100 μg/mL streptomycin, 25 μg/mL amphotericin B and 2 mM L-glutamine at 37?C in humidified air containing 5% CO2. K562 cells were treated with hemin and deferoxamine from the first day to the fifth day. The trace element levels were measured by inductively coupled plasma optical emission spectrometry. Treatment of K562 cells with hemin resulted in an increase in the levels of selenium on fifth day compared with first day. No differences were observed in selenium levels of the control group compared with the hemin-induced group. Also there were no significant differences observed in the zinc levels of control cells compared with deferoxamine- and hemin-induced cells. Iron levels of hemin-induced cells were decreased on the fourth day com-pared with the third day. On the third day, iron levels of hemin-induced cells were significantly increased compared to the control group. Our observations suggest that alterations of selenium and zinc levels may play a role in hemin-induction and deferoxamine-inhibition, respectively. On the other hand, iron levels may influence both in hemin-induction and deferoxamine-inhibition of K562 human leukemia cell line