TITLE:
Isolation and Characterization of Novel Microsatellite Markers in Walnut (Juglans regia L.)
AUTHORS:
Fatemeh Najafi, Mohsen Mardi, Baratali Fakheri, Seyed Moustafa Pirseyedi, Nafiseh Mehdinejad, Maryam Farsi
KEYWORDS:
Isolation; Microsatellite; Juglans regia L.; Polymorphism; SSR Markers
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.3,
February
20,
2014
ABSTRACT: Thirteen microsatellite markers were isolated and characterized from Juglans
regia L. Successful polymorphism of the 13
primer pairs was observed in various genotypes of J. regia. The number
of polymorphic alleles ranged from 2 to 4 (with an average of 4.35). The
polymorphic information content values ranged from 0.47 to 0.88 (with an
average of 0.69). TC/AG and GAA/CTT class of repeats were the most abundant
di-nucleotide and tri-nucleotide repeats, respectively.