TITLE:
A comparison of methods used to determine the oleic/linoleic acid ratio in cultivated peanut (Arachis hypogaea L.)
AUTHORS:
Kelly D. Chamberlin, Noelle A. Barkley, Barry L. Tillman, Jack W. Dillwith, Robin Madden, Mark E. Payton, Rebecca S. Bennett
KEYWORDS:
High Oleic Acid; Peanut; Fatty Acid; Gas Chromatography; Capillary Electrophoresis; Near Infrared Spectroscopy; Real Time PCR
JOURNAL NAME:
Agricultural Sciences,
Vol.5 No.3,
February
19,
2014
ABSTRACT:
There is an increasing
demand in the peanut industry for high oleic peanuts and also for the
incorporation of the high oleate trait into newly released varieties. Early
generation screening of breeding lines for high oleic acid content greatly
increases the efficiency of developing new peanut varieties. The objective of
this study was to compare the accuracy of methods used to classify individual
peanut seed as high oleic or not high oleic. Results from capillary electrophoresis
(CE), two variations of near-infrared reflectance spectroscopy (NIRS), and
real-time polymerase chain reaction (RT-PCR) genotyping, were compared with the
traditionally accepted reference standard results from gas chromatography (GC).
Three hundred and seventy-four (374) seeds, spanning twenty-three (23) genotypes
and all four peanut market-types (runner, Spanish, Valencia and Virginia), were
individually tested by each method. Percent accuracy levels for rating
individual seed as high oleic (H) ranged from 97.4% (NIRS) to 99.5% (CE). All
of the methods examined in this study carry only a minor risk for
miss-classification (loss of material) and are suitable for use by peanut breeding
programs in early generation breeding line screening.