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• TITLE: Detection of Helicobacter pylori in dental plaque of mexican children by real-time PCR

  AUTHORS: J. A. Valdez-Gonzalez, P. C. Mares-Moreno, M. J. Kowolik, J. Vargas-Villlarreal, F. Gonzalez-Salazar, M. A. De la Garza-Ramos

  KEYWORDS: Helicobacter pylori; Oral Pathology; Children; Real Time Polymerase Chain Reaction; Anaerobic Bacteria

  JOURNAL NAME: Health, Vol.6 No.4, February 18, 2014

  ABSTRACT: Dental plaque in adult patients is well identified as a reservoir for Helicobacter pylori. This question still remains unclear in children. The aim of this study is to identify the presence of this bacterium in dental plaque of Mexican pediatric patients, using Real Time Polymerase Chain Reaction (qPCR). Forty patients from 2 to 11 years without dyspeptic symptoms were enrolled. Samples were collected from the subgingival space of the lingual side of the lower molars and cultured in selective medium. Therefore, qPCR analysis was conducted. According to the results obtained in this study, it was found that 35% of the pediatric population who participated tested qPCR positive for the presence of H. pylori in dental plaque samples. No significant associations were detected among isolation rate by gender or age. We found that dental plaque may be a reservoir for H. pylori. However, more research is needed to establish the way of the infection of pediatric population.