TITLE:
A Green Approach towards the Synthesis of Enantio Pure Diols Using Horse Radish Peroxidase Enzyme Immobilized on Magnetic Nanoparticles
AUTHORS:
Seelam Siva Deepthi, Ernala Prasad, Basireddy Venkata Subba Reddy, Bojja Sreedhar, Adari Bhaskar Rao
KEYWORDS:
Magnetic Nanoparticles; Horseradish Peroxidase; Immobilization; Vicinal-Diols
JOURNAL NAME:
Green and Sustainable Chemistry,
Vol.4 No.1,
February
14,
2014
ABSTRACT: Enantiopure
epoxides and their corresponding chiral vicinal diols serve as valuable
intermediates in the synthesis of
biologically active pharma and agro-compounds and also value added fine
chemicals. Biocatalysts are well known for their selective hydrolysis of
racemic epoxides to give optically pure chiral diols. This study highlights an
efficient process of synthesis of chiral vicinal diols in good yields and enantioselectiviy using horse radish peroxidase enzyme immobilized on the amine functionalized magnetic
nano particles (Fe3O4 nanoparticles) as enzyme carriers. It also facilitates separation of
MNP-immobilized enzymes by applying external magnetic field. The immobilization
of magnetic nano particles was confirmed by transmission electron microscope
(TEM) and scanning electron microscope (SEM). The MNP-immobilized peroxidase
enzyme improved stability of the enzyme and has shown broader substrate specificity in enantioselective
hydrolysis of racemic epoxides, under mild and environmentally friendly
conditions. The methodology MNP-immobilized enzyme developed in the synthesis
of chiral diols has a potential for use in large-scale applications.