TITLE:
Aquaporin 8 mRNA expression after intestinal resection in rat
AUTHORS:
Yu Koyama, Hitoshi Kameyama, Jun Sakata, Takashi Kobayashi, Masahiro Minagawa, Shin-ichi Kosugi, Tadashi Yamamoto, Kohei Akazawa, Toshifumi Wakai
KEYWORDS:
Aquaporin 8; Short Bowel; Subtotal Colectomy; RNase Protection Assay; QuantitativeRT-PCR
JOURNAL NAME:
Open Journal of Gastroenterology,
Vol.4 No.2,
February
12,
2014
ABSTRACT: Backgrounds:
Aquaporins (AQPs), the mammalian water channels, have been localized in various
organs, including the gastrointestinal (GI) tract. We examined AQPs
expression in rat models of massive intestineal resection to determine the
functions of AQPs in the GI tract. Methods: Female Sprague-Dawley rats (n = 15)
underwent 90% resection of the small intestine, and Female Wistar-Kyoto rats
(n = 10), received subtotal colectomy, and were sacrificed following the
operations. RNase protection assay and quantitative reverse
transcription-polymerase chain reaction (RT-PCR) were performed to measure the
AQPs mRNA expression in the GI tract.
Immunohistochemistry was performed to confirm AQP8 protein expression.
Results: AQP8 mRNA expression (mean ± standard error), was enhanced in the
jejunum of the short bowel rats at days 7 and 14 (37.6% ± 1.4% and 18.5% ±
2.4%, respectively, p