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Koyama, Y., Yamamoto, T., Tani, T., Nihei, K., Kondo, D., Funaki, H., Yaoita, E., Kawasaki, K., Sato, N., Hatakeyama, K. and Kihara, I. (1999) Expression and localization of aquaporins in rat gastrointestinal tract. American Journal of Physiology, 276, C621-C627.

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• TITLE: Aquaporin 8 mRNA expression after intestinal resection in rat

  AUTHORS: Yu Koyama, Hitoshi Kameyama, Jun Sakata, Takashi Kobayashi, Masahiro Minagawa, Shin-ichi Kosugi, Tadashi Yamamoto, Kohei Akazawa, Toshifumi Wakai

  KEYWORDS: Aquaporin 8; Short Bowel; Subtotal Colectomy; RNase Protection Assay; QuantitativeRT-PCR

  JOURNAL NAME: Open Journal of Gastroenterology, Vol.4 No.2, February 12, 2014

  ABSTRACT: Backgrounds: Aquaporins (AQPs), the mammalian water channels, have been localized in various organs, including the gastrointestinal (GI) tract. We examined AQPs expression in rat models of massive intestineal resection to determine the functions of AQPs in the GI tract. Methods: Female Sprague-Dawley rats (n = 15) underwent 90% resection of the small intestine, and Female Wistar-Kyoto rats (n = 10), received subtotal colectomy, and were sacrificed following the operations. RNase protection assay and quantitative reverse transcription-polymerase chain reaction (RT-PCR) were performed to measure the AQPs mRNA expression in the GI tract. Immunohistochemistry was performed to confirm AQP8 protein expression. Results: AQP8 mRNA expression (mean ± standard error), was enhanced in the jejunum of the short bowel rats at days 7 and 14 (37.6% ± 1.4% and 18.5% ± 2.4%, respectively, p