TITLE:
Influence of Bavistin, Cefotoxime, Kanamycin and Silver Thiosulphate on Plant Regeneration of Solanum viarum (Dunal)—An Important Anticancer Medicinal Plant
AUTHORS:
M. D. Mahadev, Chandra Sekhar Panathula, C. V. Naidu
KEYWORDS:
Solanum viarum; Bavistin; Cefotoxime; Kanamycin; Silver Thiosulphate; Plant Growth Regulators
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.3,
February
11,
2014
ABSTRACT: The effects of bavistin, cefotoxime and kanamycin
along with the ethylene inhibitor silver thiosulphate on in vitro regeneration using the axillary buds of Solanum viarum (D.) have been investigated. Axillary bud explants when cultured on MS medium
supplemented with bavistin (100 mg/l) maximum number of shoots (4.21 ± 0.83) were observed. In a
separate experiment, bavistin along with growth regulators
such as BAP and TDZ was added to MS medium containing sucrose
(3%). Maximum number of shoots was obtained (5.55 ± 0.28) in MS medium supplemented with
bavistin (100 mg/l) and BAP (2.0 mg/l). Cefotoxime and kanamycin (10-150 μM/L) were added to MS medium supplemented with BAP (2.0 mg/l) with sucrose (3%) having shown the maximum shoot
regeneration (4.38 ± 0.45 and 3.4 ± 0.48) at 80 μM/L and 50 μM/L respectively. Similarly the
regeneration medium was supplemented with (5.0-100 μM/L) of STS. Maximum number of
shoots (4.75 ± 0.43) was seen with 40 μM/L. These plantlets
were further maintained for root emergence on a rooting medium supplemented
with growth regulators such as (0.5-2.0 mg/l) IAA, IBA and NAA. In MS medium supplemented with IBA (1.0 mg/l), maximum number of roots (24.3 ± 0.31) was seen with 100% regeneration.
The rooted plants were acclimatized and transferred to field plots with (95%)
of plant surviving in the field.